Tens of thousands of Czechs protested against Prime Minister Andrej Babis on Tuesday after prosecutors reopened a case into alleged subsidy fraud by the leader and Brussels declared a conflict of interest between his businesses and political role.
Separately, an audit by the European Commission, the European Union's executive, leaked to Czech media last week, showed the Commission saw Babis in conflict of interest because he still had control over Agrofert, a conglomerate of hundreds of firms he had built over the past two decades and put into trust funds in 2017.
The protest group, set up by students, had brought a quarter of a million people to the streets in Prague twice earlier this year in the biggest demonstrations of the country's 30-year post-communist era.
The protest on Tuesday at Prague's central Wenceslas Square was smaller, numbering several tens of thousands according to a Reuters' estimate, and 60,000 according to the organisers.
Despite the protests, Babis's populist ANO movement remains by far the most popular political party with around 30 percent of the vote, drawing support among older voters and regions outside the capital.
His firms span farming, food processing, chemicals, and media, and are among the central European country's main recipients of various EU money, from farm subsidies that are given per hectare or per animal to project-based finance for innovation and environmental investments.