Diego Costa (L), Alvaro Morata (R). Photograph:( Reuters )
Atletico have never beaten Barcelona in the top flight since Simeone took charge in December 2011.
Atletico Madrid announced on its official Twitter account on Saturday that strikers Alvaro Morata and Diego Costa were both passed fit after recovering from injuries ahead of the crucial Barcelona clash.
Atletico stated, "Good news! Diego Costa and Alvaro Morata have received the medical all-clear and will be available for Barca-Atleti."
Both trained normally on Friday as Diego Simeone finalised his plans for the meeting with LaLiga Santander's leaders on Saturday.
Costa hurt his hamstring in last Saturday's 4-0 win at Alaves and missed Tuesday's 2-0 win over Girona although medical tests ruled out a torn muscle. Morata sprained his ankle against Girona and looked certain to miss the game with Barca.
Atletico trail Barca by eight points at the top of La Liga with eight games remaining and realistically have to win at the Nou Camp, where they have not earned a victory since 2006, to stay in the title race.
Atletico, however, have never beaten the Catalans in the top flight since Simeone took charge in December 2011.
"That we've never won at the Nou Camp (actually) makes me very optimistic," Simeone told reporters on Friday.
"If we win tomorrow and then we don't win the next games, it counts for nothing. Tomorrow we have to show the identity which this team has," he added.